It depends on what you call "uptake" and on the volume used for intranasal or intratracheal instillation (usually 50 to 100 µl in mouse models). Binding of influenza virus to its receptors is fast (a matter of minutes in small volumes). Internalisation follows and, as correctly stated in a previous answer, mRNA transcription is obvious after 2 hours. Large volumes used for inoculation may result in a less synchronous and delayed infection process.
It takes less than 30 minutes. The size of particle is crucial, and it has to be smaler than 12 and bigger than 3 microns. Macrophages (alredy present in lungs - sesile) will do the job.
Within minutes it is being taken up for a mucosal immunity to be developed
Thanks everyone for your answers. Do you think that staining the epithelial cells of lungs of mice (lungs removed within 20-30 mins of pulmonary delivery) would result in detectable antigen in these cells?
We give the vaccine only by IM injection or by intranasal route. The latter is a live virus. The former is killed. We do not vaccinate humans by the intrapulmonary route in the US
Carl White, MD
This should be in a matter of few minutes. The attachment to the available receptor sites of the URT cells are specific and the reaction is chemical. So, as soon as the receptor cites of the cell and the attachment sites of the virus (influenza) in this case is haemagglutinin comes in contact starts like an electric switch. I wish some one (as I am a retired person with no lab) should attempt to look into.
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