1. How Long does it Take for Tamoxifen to activate Cre and knock out the gene of interest
2. What would the dosing regimen be for rats/mice?
I am designing an experiment where I will be knocking out 5-HT2A receptors in a cell type-specific manner before psilocybin administration to determine the necessity of 5-HT2A receptors in psilocybin's neuroplastic effects.
Which cells do you want to reach ?
It is extremely important to know the target location. e.g. blood-brain barrier.. 1-2 dosages are sufficient, intracerebral (e.g. astrocytes, neuron) can take a while.
Depending on your animal facility you will have difficulties due to quarantine time
of the tamoxifen.
thank you for the response. I would want to reach cells in the prefrontal cortex and the hippocampus
Typically, it is recommended that tamoxifen is resuspended with corn oil to a final concentration of 20 mg/ml and injected intraperitoneally at a dose of 75mg/kg body weight for 5 consecutive days. For Cre characterization work induced by tamoxifen, there is a 7-day waiting period between the final injection and necropsy/histological analysis.
Also, tamoxifen can be fed by garage to rats/mice at doses of 130~160mg/kg body weight. Regimen for maintaining administration and the waiting time after the last administration are consistent with intraperitoneal injection.
- Badges
- Science method