How long do I have to lyse cells in "Promega Cell Culture Lysis Reagent" for best results?

Christoph Gassner-Schneckenleithner @Christoph-Gassner-Schneckenleithner

15 December 2012 1 7K Report

I work with the T47D-KBluc cell line (human breast cancer, ATCC No: CRL-2865), and want to perform the Promega Luciferase Assay System. Therefore I need to lyse the cells with 20µl/well CCLR (1x), but the protocol doesn't provide details on the time required for sufficent cell lysis. Has somebody made experiences with CCLR or recommendations?

Hiroshi Fukasawa

In our lab, we lyse cells with passive lysis buffer for 10 min by using seasaw rocking mixer. That always works well enough.

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