Christoph Gassner-Schneckenleithner

2 Questions 1 Answers 0 Followers

Questions related from Christoph Gassner-Schneckenleithner

Has anybody experience with a High-Dose-Hook-Effect caused by 17-beta-estradiol in a hormone sensitive cell line?

I work with the T47D-KBluc cell line, which is stably transfected with a triplet ERE (estrogen-responsive elements)–promoter–luciferase reporter gene construct. Therefore the cell line is...

17 December 2012 3,963 2 View

How long do I have to lyse cells in "Promega Cell Culture Lysis Reagent" for best results?

I work with the T47D-KBluc cell line (human breast cancer, ATCC No: CRL-2865), and want to perform the Promega Luciferase Assay System. Therefore I need to lyse the cells with 20µl/well CCLR...

15 December 2012 7,310 1 View