This morning (Tuesday) I started blocking some coated ELISA plates but I was unable to acquire sufficient sample to actually do the ELISAs today. They blocked for 2 hours at 37C before I moved them to 4C. Can they sit at 4C until tomorrow or preferably Thursday? I can remake the plates if necessary, would just be kind of a pain in the ass and it would be nice not to have that 2 hour wait period again in the future. If it matters, my blocking buffer is 1% BSA and 0.05% Neonate 20 in PBS. Thanks!
The short answer is "yes". you can keep ur plate coated with blocking buffer until tomorrow or even more hours, I have done it before without any problem.
If in hurry, why block for two hours? But once coated & blocked, plates can be stored in a fridge for a long period until use.
The most basic blocking buffer contains 1 % BSA or milk proteins dissolved in PBS. Usually, 150 ul of blocking buffer is added to the well to incubate for one hour (sometimes 1.5 hours) at 37 C in order to fully block the plate.
- Badges
- Science topic
- Similar topics
- Physical Sciences
- Materials Science
- Plating