23 January 2020 3 357 Report

I intend to use maleimide chemistry to attach a short peptide with a terminal cysteine to the N-terminal domain of another protein. Do I need to pretreat the short peptide with a reducing agent to eliminate inter-peptide disulfide bonds, or will this not be favored?

Thanks!

More Ragan Pitner's questions See All
Similar questions and discussions