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Questions related from Ragan Pitner
Hi all, I recently ran a restriction digest check of several of my plasmids and one of them was weird. All the plasmids in this picture received the same restriction enzymes and are all loaded...
13 July 2021 2,962 17 View
This morning (Tuesday) I started blocking some coated ELISA plates but I was unable to acquire sufficient sample to actually do the ELISAs today. They blocked for 2 hours at 37C before I moved...
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Hello, For one of my experiments, I enriched cells by staining them with a PE-conjugated antibody and performing a positive anti-PE enrichment to obtain a population that was 64% PE+. After 4...
18 April 2020 2,576 2 View
I'm going to do some OT-II/DC co-culture experiments and was wondering whether it would be feasible for me to do a few big CD11c DC enrichments, freeze them, and then use them in my co-culture...
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I intend to use maleimide chemistry to attach a short peptide with a terminal cysteine to the N-terminal domain of another protein. Do I need to pretreat the short peptide with a reducing agent to...
23 January 2020 316 3 View