As the implantation of mesenchymal stem cells is normally done at a place other than where these are cultured, there is time gap in implantation of MSCs for treatment. What is the time period for which we can maintain cells at room temperature without having any deleterious effect on cells?
A simple experiment could give you the answers for this question.
I would suggest that you should perform a time lapse experiment where you can keep cells in room temperature in the medium you would inject the cells in..eg;Normal saline with or without ringer lactate.You could monitor the cell viability by a simple trypan blue exclusion experiment maybe using time points from 1 hour to 10 hours.
I would like to think the cells will remain 90% viable even after 3 hours at room temperature.The medium in which the cells are kept in will play a major role in their viability.
Regards
Charan
Most of the normally used media like serum, plasma keep cells viability upto 90% but significantly decrease beyond.
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