How to make sure each cavity modes with same E-field distribution in COMSOL simulation?

In COMSOL Application Library, there is case about waveguide adapter. But there are some questions: 1) In boundary mode analysis, it just set to find only 1 mode and the S-parameter is...

12 January 2021 9,383 2 View

Where to get per-unit ecosystem services value coefficients for cities?

Per-unit ecosystem services value coefficients are wisely used in ecosystem services research, which is usually in the unit of "money per area for certain ecosystem type/services". The ecosystem...

06 January 2021 4,230 3 View

How to set up rheological model (CA and CAM) ?

how to set up asphalt rheological model (CA and CAM) ? How to build EXCEL?

23 September 2020 7,692 2 View

How to detect protein from dying cells?

Hello, experts. I have been struggling during my Western blot. Briefly, I transfected my gene into two different breast cancer cell lines. The introduction of my gene triggered cell death in one...

03 September 2020 8,799 3 View

What's the difference in antibodies used in ELISA,FACS and IHC?

Hello, I have a question about antibodies used in ELISA, FACS, and IHC. Assuming that an antibody is not labeled or conjugated, what's the difference among them? Can I treat those antibodies in...

27 July 2020 716 11 View

How can I generate Deek's funnel plot in R software, not in STATA?

I am conducting Diagnostic test accuracy meta-analysis. For publication bias, I want to conduct Deek's funnel plot test. I am using R software with mada ant meta packages. However, I found that...

16 May 2020 4,368 1 View

How to grow more BL21 auxothrophic strain cells?

Dear all, I have just purchased a BL21 auxothrophic strain (RF12) from Addgene. The cells come in the form of agar stab. I intend to grow more cells which will be ready to used for protein...

11 May 2020 8,384 3 View

Do you know a lipo battery that is smaller than 10mmX9mmX3mm??

Hi everyone, I'm looking for a small/tiny battery that can generate over 3.2V for biocompatible electronics. Currently, I'm using a 3.7V 12mAh Lipo battery and its size is 10mmX9mmX3mm (WLH). I...

08 March 2020 8,641 2 View

Does anybody know how to draw a pie chart with multiple layers?

I found this graph from literature and it's an excellent way to illustrate similar types of ideas in my opinion. I would like to draw this type of graph in my work but I couldn't find the proper...

14 February 2020 5,933 2 View

How to fit the Gaussian curve based on the moment of power spectrum?

I want to make a spectral correction curve of my light source to resemble the Gaussian spectrum. This literature said, ".. The dashed curve is a Gaussian fit with a center wavelength of 833 nm...

20 January 2020 8,950 2 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Looking for genomic DNA extraction kits from mouse tissue (centrifuge-based, not spin-column)?

Hello. I am looking to extract genomic DNA from mouse brain and lungs and I need a kit that is not spin-column based because I need to scale up the solution amounts for 400mg tissue (most cap at...

25 February 2021 155 2 View

Has anyone tried to scan fluorescence IHC slides with microarray scanner?

I have an old slide microarray scanner (VIDAR Revolution 4200) and would like to give it a "second life", as IHC scanner array (fluorescence dyes), FISH over tissue or similar. Has anyone tried...

24 February 2021 9,210 1 View

Recommendations on homogenizers for RNA isolation?

I will be performing RNA isolation with frozen lung biopsy samples and am in the market for a tissue homogenizer. I am wanting a homogenizer that will fit in a 1.5ml tube. I was looking at the...

24 February 2021 3,623 3 View

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22 February 2021 9,932 11 View

How to locate and collect the pineal gland in the amphibian brain?

Our team has been trying to locate and collect the pineal gland in the American bullfrog's brain (Lithobates catesbeianus) for some time now. However, we don't have a standardized protocol for...

18 February 2021 3,453 4 View