Exosomes in our hands are stable as defined by nanosight analysis. However, by Western blot analysis, exosome surface proteins appear to disappear in a time-dependent manner. Has anyone else observed this phenom and how have you handled it if you have?
Alexander Sasha Vlassov
we never did detailed "longevity" studies, but ~ 2 months at -80C did not harm the surface proteins, according to Westerns
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Dan Stratton
Depending on the freezing process, we found that they can last over a year.
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Balasubramanyam Karanam
If you snap freeze the protein in liquid nitrogen and store in liquid nitrogen they should stay stable for months
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Barbara Dale Summers
This is a question to nathan or anyone else for that matter- How did you look only at surface proteins by western blot in exosomes?
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