Have a look at the ExoELISA kit from System Biosciences. I didn't try it yet, but you might consider it useful.

We have observed a tremendous amount of exosome heterogeneity, so we have been reluctant to try the ExoELISA. It assumes that all of your vesicles contain a specific marker and there is no variability of the exosome composition from difference samples/ sources. For example, studies by our group and others (including Clothilde Thery) have shown by ImmunoEM that a high percentage of purified exosomes won't label with antibodies against the classic exosome markers (eg, CD63, CD9, etc).

We just coordinated a group purchase for the IZON qNano. It is less user friendly than the NanoSight, but priced at ~1/3 of the cost of a Nanosight instrument. It was a good buy.

I would not use the IZON as it can't differentiate smaller particles. You could try either the Guava Millipore flow cytometer (40nm) or a NanoSight? At a conference on extracellular vesicles, the flow cytometer was far superior.

Don't forget that exosomes clump together and will need separating.

Thanks Dan-this is very helpful as I have felt that the Nanosight out of all the instruments we have tried out is the most superior.

I personally have used IZON, Nanosight and Guava and do prefer the Guava Flow Cytometer. Before buying a NanoSight get some clarification about the resolution power, as I believe that the nanosight can only view metallic particles smaller than 40nm. Good luck.

Thanks Dan. Can you provide a little more info on the Guava that you have and the specific protocols you use?

If you have an email I can send you comprehensive info.

Thanks Dan

[email protected]

Hi Dan,

Can you please forward me the information on Guava flow cytometer as well?

[email protected]

Thanks a lot!

Hi Dan

I would be very interested in learning more about your application of the guava for exosome analysis. Specifically, are using it to count/identify free particles? Any information you could provide would be greatly appreciated.

[email protected]

thank you

amedeo

Can someone tell me, that for sending blood plasma samples for nanosight analysis from India to UK...what should be the storage condition?

Hi Dan, 

Could you please forward me information on Guava flow cytometer for exosome analysis. My email is [email protected]

Thanks!

iZon is somewhat cheaper; particle metrix more expensive. i really like Nanosight, they have all bells and whistles added now. no easy way to count/size nanoparticles with any other, simpler instruments

Is anyone using iZON qNano for zeta analyser?

I need to measure the zeta potential of my liposome samples and I need to know how to operate iZON qNano and how to prepare my sample?

Thank you

Hi Dan, I know you posted your first message 4 years ago, so I hope you still check your researchgate answers. As others did, I'm also very interested in what you stated about exosome analysis with Guava flow cytometer. In our lab we have a Guava easyCyte 6-2L and I want to use it to analyze EV preps (both microvesicles and exosomes). Could you please forward me too comprehensive info on how you perform EV analysis on your Guava? If anyone here has that info from Dan and share it with me I'd appreciate it a lot too. My e-mail is [email protected]