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Questions related from Barbara Dale Summers
Been trying to find a good antibody for alpha ENaC for mouse kidney lysate. We have not been getting very good results. Anybody know of a good antibody to buy?
06 June 2019 7,850 2 View
I know that you can measure hydroxyproline from tissues which have a greater amount of collagen than cells. However I need to measure hydroxyproline from cells grown from a 12 well plate...
06 June 2018 1,303 3 View
I am trying to do double staining on mouse tissue but have not found any good fluorescent method for my target antibody. SO I would like to do both HRP and FITC on the same slide. I have used each...
10 October 2017 3,583 2 View
We have tried the armenian hamster anti mouse and a secondary for rhodamine to use for fluorescence but it didn't work. We wanted fluorescence so we could double stain with another antibody .
10 October 2017 8,593 1 View
I have tried a few times and have gotten many bands- probably do to the various isoforms it has. Any suggestions? I read that you need to run a nondenatured gel. However, can you get away with...
02 February 2014 6,165 2 View
I am getting variable numbers. I have been sonicating with a probe sonicator after cutting tissue into small 2 mm pieces. After rotating ON in the acetic acid/pepsin buffer(0.5 M ,2.5 mg/ml) my...
01 January 2014 4,803 1 View
I use mast cells lysates in both RIPA or PBS that have been sonicated and centrifuged. Is it the membrane proteins in the lysates?
01 January 1970 5,147 4 View
