Sepideh Sadighbathi

2 Questions 3 Answers 0 Followers

Questions related from Sepideh Sadighbathi

How long a transformed bacteria with correct 3HR and 5HR assembly can be stored in fridge?

I cloned 3HR and 5HR of p.falciparum on a plasmid and the next step is gRNA cloning. I did colony PCR for verification it is perfect but the thing is after taking the toothpeak of the colony in...

13 March 2024 5,690 2 View

Why do I get three bands after restriction digestion?

I ran restriction digestion on my plasmid just to verify my inserts and send for sequencing. my backbone plasmid is PL6 with 9843 bp containing a 3HR (877bp) and 5HR (782). My enzymes for 3HR is...

08 February 2024 7,967 4 View