How to analyze bacterial inhabitation?

Hello, everyone. I found the bacteria I cultured can be inhabitated by another bacteria when I culture then together in the plate. Now, I want to analyze which metabolite contribute to the...

11 December 2018 4,934 2 View

Is there any reference about how may sample do you need for metabolomics analysis?

For metabolimics analysis, sample repeat is necessary, but how to know how ,any samples do I need is enough for analysis? For example, microorganism, plant or tissue?

06 July 2018 5,006 3 View

Can I use relative quantitative data analysis of NMR for multiomics analysis?

When I analyze NMR data for metabolomics combined with other omics, can I use relative quantification data but not absolute quantification to do multi-omics data analysis?

06 July 2018 764 5 View

Anyone know the composition of beef extract and yeast extract?

anyone know the composition of beef extract and yeast extract

31 December 2017 2,956 5 View

How to avoid sample loss when I use freeze dryer?

When I use freeze-dryer, after freeze at -80C for 5 or 7h, drying it but there are always sample loss after drying, some of the dried sample get out of the glass tube. How to avoid the loss?

02 March 2017 7,954 7 View

20 amino acids analyze with GC MS?

How to analyze 20 amino acids with GC MS? I cannot separate proline-isoleucine peak and cannot detected arginine, glutamine peaks.

01 January 1970 8,417 1 View

Derivatization of amino acids analyzed by GCMS?

01 January 1970 2,379 11 View

How to analyze glyoxylic acid, pyruvic acid with GC MS

I want to analyze TCA cycle, but pyruvic acid, glyoxylic acid cannot be detected. My GC MS is 7890A-5957C, HP-5MS column, MTBSTFA as derivatization reagent, Anyone can give some advice?

01 January 1970 1,224 3 View

Anyone know muma package in R language?

I am a beginner in R language and I want to analyze metabolomics data with R. Recently I found an R package "muma" which is for metabolomics data analysis but I don't know how to use that package....

01 January 1970 9,141 6 View

Does the pressure(-600 mmHg) affects pH?

When I conducted the degassing experiment using decompression under anaerobic condition(and I used ferrous based chelating agent), the oxidized ferric were observerd. So, could you tell me the...

03 March 2021 4,892 5 View

What solvent can I used for Paal Knorr reaction between 6-amino hexanoic acid and 2.5 hexanedione?

I am try to make the Paal Knorr reaction between 2.5 hexnedione and 6-amino hexanoic acid, my problem is type of solvent to use, because 6-hexaminohexanoic acid is soluble in water but I am not...

02 March 2021 4,443 2 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

How can I convert .vesta file to a .cif file?

I've used the "export data" option in Vesta software to convert my unit cell into a cif file however when I use that cif in atomsk it shows error such as "can't read the position of atom 0". If I...

28 February 2021 4,966 3 View

What is the exact mechanism of the antimicrobial effect of local anesthetics?

The antimicrobial effect of local anesthetics has been known for more than 50 years, but it still seems to have an unclear mechanism of action. which bacteria? which concentration? etc etc. ....

27 February 2021 4,450 2 View

I want to performe MALDI-TOF MS for bacterial identification, I would appreciate it if anyone can tell me the matrix solution preparation protocol ?

based on what I've studied on multiple papers, for peptide and protein identification Alpha-cyano-4-hydroxycinnamic acid (CHCA) is an appropriate matrix to use, I also know that the CHCA powder...

26 February 2021 6,799 3 View

Which database would give me this list of chemical components?

Dear colleagues: I would like to know if anyone knows of a database that gives me the parameters pH, K, K, Mg, P, C, N, Zn, Cu, Ni and Cd of common foods. I find a lot of information of proteins,...

26 February 2021 5,525 3 View

No amplification data for qPCR on MxPro?

I have set up a qPCR run on MxPro with SYBR green and ROX as a reference, however, when I go to the analysis section the program seems to only collect data for the dissociation curves and not the...

25 February 2021 8,039 1 View

Uncertianiny in using dry or wet basis in mixing a sediment with fuel oil?

Hello I'm currently working on a topic related to studying the anaerobic biodegradation of TPH in marine sediments. In this work, sediment should be mixed with fuel oil to a concentration of 20 g...

24 February 2021 9,839 3 View

Advice on growing lactobacillus species in the lab?

Hello everyone, For part of my PhD project, I'm hoping to grow some lactobacillus species in the lab so that I can create standard curves for a qPCR quantification of a faecal sample. However, I...

24 February 2021 3,157 6 View