pH of medium would be decreased, anyone know how cell export lactic acid into medium?
Hello, everyone. I found the bacteria I cultured can be inhabitated by another bacteria when I culture then together in the plate. Now, I want to analyze which metabolite contribute to the...
11 December 2018 5,011 2 View
For metabolimics analysis, sample repeat is necessary, but how to know how ,any samples do I need is enough for analysis? For example, microorganism, plant or tissue?
06 July 2018 5,113 3 View
When I analyze NMR data for metabolomics combined with other omics, can I use relative quantification data but not absolute quantification to do multi-omics data analysis?
06 July 2018 832 5 View
anyone know the composition of beef extract and yeast extract
31 December 2017 3,061 5 View
When I use freeze-dryer, after freeze at -80C for 5 or 7h, drying it but there are always sample loss after drying, some of the dried sample get out of the glass tube. How to avoid the loss?
02 March 2017 8,108 7 View
How to analyze 20 amino acids with GC MS? I cannot separate proline-isoleucine peak and cannot detected arginine, glutamine peaks.
01 January 1970 8,486 1 View
When I analyze 20 amino acids with GCMS, I use MSTFA to react with sample, but there are some combined peaks cannot be separate. Like proline-Isoleucine, and i cannot detect tryptophan and...
01 January 1970 2,509 11 View
I want to analyze TCA cycle, but pyruvic acid, glyoxylic acid cannot be detected. My GC MS is 7890A-5957C, HP-5MS column, MTBSTFA as derivatization reagent, Anyone can give some advice?
01 January 1970 1,315 3 View
I am a beginner in R language and I want to analyze metabolomics data with R. Recently I found an R package "muma" which is for metabolomics data analysis but I don't know how to use that package....
01 January 1970 9,216 6 View
I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...
07 August 2024 6,034 3 View
Hello What should be done to separate and identify organic acids in HPC when their RetTime is the same?Like oxalic acid with Propanoic Acid.or acids that have a very close RetTime.
07 August 2024 8,782 3 View
Hello, I suspect that a molecule I am using is causing bacteria to experience osmotic shock. What chemical properties should I look for to compare this capability with those of other substances?...
06 August 2024 977 1 View
Palmitic acid presence in aqueous fraction
05 August 2024 8,624 4 View
There are anaerobic gas pak jars and environment providers in the laboratory that provide anaerobic environmental conditions for the growth of anaerobic bacteria. However, there is no anaerobic...
05 August 2024 4,945 3 View
I have incorporated the dispersion effect for the OPBE functional by specifying the S6, SR6 and S8 parameters for GD3 in Gaussian G09 package. For this purpose, I have defined the environment...
05 August 2024 1,494 2 View
I used humic acid at 0.044 g/kg soil in my pot experiment. But finally, I have to recommend kg/ha. Each pot's soil weight was 11 kg. What is the solution?
02 August 2024 7,186 6 View
I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...
30 July 2024 6,420 1 View
We are working on biopolymeric hydrogels. Our system is highly viscous and sticky, and the gel formed are high in strength. We are unable to use pH electrode and pH strip. Please suggest an easy...
30 July 2024 942 2 View
Hello everyone! How can i prepare EDTA buffer solution (pH 8.2) to determine GSH levels? Thank you in advance.
29 July 2024 4,374 1 View