20 amino acids analyze with GC MS?

01 January 1970 1 8K Report

How to analyze 20 amino acids with GC MS? I cannot separate proline-isoleucine peak and cannot detected arginine, glutamine peaks.

Xiaofang Yuan

80 μl MSTFA was used to derivatization, heat at 70 ℃ for 1h, and analyze with GC MS, splitless, HP 5-MS column, temperature is 80℃，2min, 5℃/min to 230, 10℃/min to 300, hold for 5min.

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