How to analyze 20 amino acids with GC MS? I cannot separate proline-isoleucine peak and cannot detected arginine, glutamine peaks.
80 μl MSTFA was used to derivatization, heat at 70 ℃ for 1h, and analyze with GC MS, splitless, HP 5-MS column, temperature is 80℃,2min, 5℃/min to 230, 10℃/min to 300, hold for 5min.
Hello, everyone. I found the bacteria I cultured can be inhabitated by another bacteria when I culture then together in the plate. Now, I want to analyze which metabolite contribute to the...
11 December 2018 4,934 2 View
For metabolimics analysis, sample repeat is necessary, but how to know how ,any samples do I need is enough for analysis? For example, microorganism, plant or tissue?
06 July 2018 5,006 3 View
When I analyze NMR data for metabolomics combined with other omics, can I use relative quantification data but not absolute quantification to do multi-omics data analysis?
06 July 2018 764 5 View
anyone know the composition of beef extract and yeast extract
31 December 2017 2,956 5 View
pH of medium would be decreased, anyone know how cell export lactic acid into medium?
11 December 2017 2,932 0 View
When I use freeze-dryer, after freeze at -80C for 5 or 7h, drying it but there are always sample loss after drying, some of the dried sample get out of the glass tube. How to avoid the loss?
02 March 2017 7,954 7 View
01 January 1970 2,379 11 View
I want to analyze TCA cycle, but pyruvic acid, glyoxylic acid cannot be detected. My GC MS is 7890A-5957C, HP-5MS column, MTBSTFA as derivatization reagent, Anyone can give some advice?
01 January 1970 1,224 3 View
I am a beginner in R language and I want to analyze metabolomics data with R. Recently I found an R package "muma" which is for metabolomics data analysis but I don't know how to use that package....
01 January 1970 9,141 6 View
How to calculate the RMSD values for a MD simulation using MOE?
07 August 2021 0 0 View
When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.
16 June 2021 0 0 View
effect of double bond on the flexibility of polymers
07 April 2021 0 0 View
I am writing to request some information for making polymer color flakes. I would appreciate the help.
17 March 2021 0 0 View
Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...
03 March 2021 8,796 3 View
I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance
03 March 2021 3,568 1 View