Is there any reference about how may sample do you need for metabolomics analysis?

For metabolimics analysis, sample repeat is necessary, but how to know how ,any samples do I need is enough for analysis? For example, microorganism, plant or tissue?

06 July 2018 5,006 3 View

Can I use relative quantitative data analysis of NMR for multiomics analysis?

When I analyze NMR data for metabolomics combined with other omics, can I use relative quantification data but not absolute quantification to do multi-omics data analysis?

06 July 2018 764 5 View

Anyone know the composition of beef extract and yeast extract?

anyone know the composition of beef extract and yeast extract

31 December 2017 2,956 5 View

How lactic acid bacteria export lactic acid from cell into medium?

pH of medium would be decreased, anyone know how cell export lactic acid into medium?

11 December 2017 2,932 0 View

How to avoid sample loss when I use freeze dryer?

When I use freeze-dryer, after freeze at -80C for 5 or 7h, drying it but there are always sample loss after drying, some of the dried sample get out of the glass tube. How to avoid the loss?

02 March 2017 7,954 7 View

20 amino acids analyze with GC MS?

How to analyze 20 amino acids with GC MS? I cannot separate proline-isoleucine peak and cannot detected arginine, glutamine peaks.

01 January 1970 8,417 1 View

Derivatization of amino acids analyzed by GCMS?

01 January 1970 2,379 11 View

How to analyze glyoxylic acid, pyruvic acid with GC MS

I want to analyze TCA cycle, but pyruvic acid, glyoxylic acid cannot be detected. My GC MS is 7890A-5957C, HP-5MS column, MTBSTFA as derivatization reagent, Anyone can give some advice?

01 January 1970 1,224 3 View

Anyone know muma package in R language?

I am a beginner in R language and I want to analyze metabolomics data with R. Recently I found an R package "muma" which is for metabolomics data analysis but I don't know how to use that package....

01 January 1970 9,141 6 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

1. What is the impact of having different scales in a survey? and how can we solve this problem before and after data collection (Literature)?

1. What is the impact of having different scales in a survey? and how can we solve this problem before and after data collection (Literature-based reflection)? Thank you for your time and for...

03 March 2021 2,870 3 View

What degree of missing data in a particular case/participant results in Expectation-Maximisation no longer working?

I'm dealing with a mediation model and am using the PROCESS module in SPSS. Due to SPSS and PROCESS being limited in the imputation methods - being unable to handle multiple imputation - the other...

02 March 2021 4,362 1 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View