How is immortal strand hypothesis acceptable?

More Satyajeet Salunkhe's questions See All

How mirna chose its target among 100s of mRNA having seed sequence?

I am not clear what is rule behind mirna and mRNA targeting pair,because each mirna target officially 100s of target. In such cases how one will validate legitimate mirna mRNA target. Assays...

03 April 2019 7,959 5 View

How to meassure primary miRNA trancript using QRT PCR?

I want to know how to design primer for primary miRNA QRT PCR

02 March 2018 343 7 View

Can I store phenol chloroform phase in trizol?

I use trizol for RNA isolation,but we store phenol choroform phase -30 , I want to know is it good to do isolation of protein later after storage -30 of the above mentioned phase or it may affect...

10 November 2017 7,550 0 View

Anyone used mirna mimics cholesterol conjugated for primary B cells?

I read recently that cholesterol conjugation remvoes need of lipofectamine and enhance efficiency of transfection of mimics.If any one has used please answer

11 December 2016 6,890 1 View

Can miRNA mimics or inhibitor used in primary cells?

I am currently working with lymphocyte ,I want to know if any one has used the miRNA mimics or inhibitor with primery cells

10 November 2016 3,458 4 View

Can i use different concentration of forward and reverse primer in real time ?

i am currently making assay of syber green based real time pcr for detecting rare isoforms.To make them specific ,can i use say 1 p mole of forward and 5 p mole of reverse primer,technically for...

06 July 2016 8,389 4 View

Can anyone provide a table for miRNA and respective gene from which it arises?

There are certain miRNA originated from the intron of genes. I could not found such tabulated form in which genes and respective miRNA are given Can anyone provide the same.

11 December 2015 1,695 3 View

In rel time PCR non specific band at higher dilution?

I am currently working with gene I had problem in that at higher dilution the non specific band is incresing non template control is clean,tried with higher temperatures, SYBER GREEN based real...

09 October 2015 6,288 3 View

What is meaning of miR17 approximately 92 cluster?

During literature search I found miR 17/92,miR-17 approximately 92, miR 17~92 I am not able to understand practical meaning of these annotations in microRNA. Can anybody help to me to understand...

09 October 2015 6,871 1 View

Can anyone help me with the interpretation of intron centric analysis file output ?

RNA seq data analysis by intron centric analysis gives insight about exon skkiping ,I have attached photo of excel file I have. Can anyone help me how to interpretate it eg...

06 July 2015 9,592 4 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

What are the emerging trends and opportunities for STEM education and careers in the online space?

10 August 2024 507 0 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is this a facetotecta nauplius?

This larva was captured using a plankton net in the Persian Gulf during the summer. I believe it may be a Facetotecta nauplius.

08 August 2024 3,746 4 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

How can I apply boundary conditions in an orthotropic steel deck numerical model using ABAQUS software?

I am trying to simulate vehicular loading on an orthotopic steel deck bridge section in ABAQUS software. The red arrow mark in the attached figure indicates the direction in which the vehicle will...

08 August 2024 719 0 View

Does crude extraction using NaOH and Tris work well with Fungi?

I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you

08 August 2024 4,733 2 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

Unusual intensity drop in some sections of chromatograms in DDA?

Hi, we have measured tryptic peptides using both DDA and DIA method on QExactive. In DDA replicates i saw unusual intensity drops occurring at the same sections of chromatograms in DDA replicates...

07 August 2024 3,218 4 View