During literature search I found miR 17/92,miR-17 approximately 92, miR 17~92
I am not able to understand practical meaning of these annotations in microRNA.
Can anybody help to me to understand this...
Since the authors have not introduced miR17~92 as something new, ~ seems to be just different annotations for the miR-17/92 cluster.
I am not clear what is rule behind mirna and mRNA targeting pair,because each mirna target officially 100s of target. In such cases how one will validate legitimate mirna mRNA target. Assays...
03 April 2019 7,959 5 View
I want to know how to design primer for primary miRNA QRT PCR
02 March 2018 343 7 View
I use trizol for RNA isolation,but we store phenol choroform phase -30 , I want to know is it good to do isolation of protein later after storage -30 of the above mentioned phase or it may affect...
10 November 2017 7,550 0 View
I read recently that cholesterol conjugation remvoes need of lipofectamine and enhance efficiency of transfection of mimics.If any one has used please answer
11 December 2016 6,890 1 View
I am currently working with lymphocyte ,I want to know if any one has used the miRNA mimics or inhibitor with primery cells
10 November 2016 3,458 4 View
i am currently making assay of syber green based real time pcr for detecting rare isoforms.To make them specific ,can i use say 1 p mole of forward and 5 p mole of reverse primer,technically for...
06 July 2016 8,389 4 View
There are certain miRNA originated from the intron of genes. I could not found such tabulated form in which genes and respective miRNA are given Can anyone provide the same.
11 December 2015 1,695 3 View
I am currently working with gene I had problem in that at higher dilution the non specific band is incresing non template control is clean,tried with higher temperatures, SYBER GREEN based real...
09 October 2015 6,288 3 View
RNA seq data analysis by intron centric analysis gives insight about exon skkiping ,I have attached photo of excel file I have. Can anyone help me how to interpretate it eg...
06 July 2015 9,592 4 View
I need to analyse microarray data ,so I need latest algorithms can any body help me to find ..
05 June 2015 7,409 0 View
How to design VN primer to attach with universal reverse primer
05 August 2024 2,116 3 View
Hello everyone, I am currently working on miRNA- mRNA interaction study. Further, I would like to do MD simulations for the miRNA-mRNA models. For the Simulation, I have modelled this complex...
24 June 2024 3,507 2 View
Hello to everyone, I'm trying to extrtact total RNA from sEVs. I made a first experiment starting from 2x10^10 sEVs quantified by NTA but the RNA obtained was very low (under 20ng/ml) Do you have...
18 June 2024 2,799 4 View
Recently, I have been trying to quantify the expression of miRNA. I have extracted the miRNA. However, I am not clear about using the cDNA synthesis kit usually there are two kits providing by...
16 June 2024 9,723 2 View
Hello everyone, I would like to know if any of you ever tried to assess the yeld of RNA extraction with the mirVana™ miRNA Isolation Kit, without phenol from ThermoFisher. I am experincing some...
06 June 2024 5,715 4 View
The size miRNA is 89 bps and the primers I have designed (using traditional method for designing gene primers) should give an amplified product of 71 bps. But I am getting product of higher...
23 May 2024 7,570 3 View
I have a question regarding target miRNA expression after transfection with antisense miRNA (miRNA inhibitor). I understand that the expression of the target miRNA will increase after mimic...
20 May 2024 5,920 4 View
Hello. Is there any webserver where I can input a set of transcription factors, genes, and miRNAs, and it can provide me with statistical data on how TFs either activate or repress genes and...
18 May 2024 5,848 1 View
Hello all. I have recently been trying to isolate total RNA after transfection of miRNA mimics and antisense inhibitors in mammalian cells. However, the RNA isolated through the trizol method...
13 May 2024 4,935 7 View
would like to work on microRNA to detect colorectal cancer. I will use the type of microRNA type micRNA124,micro143 how I can make primer design for it and how I can choose the primer ?
07 May 2024 8,812 2 View