How is EDEM (=Htm1p=Mln1) regulated?

12 May 2014 0 10K Report

EDEM is associated with the ERAD pathway of misfolded protein degradation.

Badges
Science method

More Adrien Tereygeol's questions See All

Does anyone know of another Crustacean genomes/transcriptomes database than Genbank ?

Hi everyone, I started my PhD few days ago and I'm looking for Crustacean assembly genomes (from Caridea infra order if possible but I supposed there are no more genomes except those on GenBank)...

04 June 2023 1,655 4 View

Which article come this gating strategy ?

Hello, following a computer crash without backup (don't do that) I lost a number of data, including an article from which I had drawn the gating strategy of B cell presented in attachment. Its...

18 April 2023 7,778 1 View

How to compute unstandardized latent variable scores with seminR for formative scale?

I found a way to compute unstandardized latent variable scores: 1. Calculate the std-dev for each unstandardized indicator from summary$descriptives 2. Then use the outer loadings / std-dev=x of...

15 February 2023 2,251 1 View

Does anyone have a method to get an elevation profile from a 3D model (snake scales precisely)?

Hi everyone, I have been working recently on 3D models of snake scales from Gelsight and scanning electronic microscopy and I need to have elevation profiles of these 3D models to conduct shape...

10 January 2023 1,335 3 View

How precise is X-ray fluorescence? How many replicates do you need to test whether elemental composition of soils differ between two alpine meadows?

Ecological differention among closely related species may rely on minute environmental conditions, including in the soil. In the litterature, soil preferences are usually oversimplified, e.g.,...

02 February 2022 6,623 8 View

Open source software to process and analyse Achilles tendon ultrasound images?

Dear all I am looking for an open source software that allows processing of B-mode musculoskeletal ultrasound images (Achilles tendon to be precise). I'm looking for a software that can at...

26 January 2022 1,604 7 View

How to evaluate soil porosity in organic soils?

Hello, I would like to determine soil porosity along a soil profile (0-60cm) with >30% of organic matter (peatland soil). Does someone know how to do this? Any references? Thanks in...

27 September 2021 3,052 2 View

How to perform intramuscular EMG electrode placement with electrical nerve stimulation ?

We are conducting a study with intramuscular EMG electrode inserted in the neck, but the targeted muscle is a bit difficult to locate. To be sure that the electrode is actually inserted in the...

28 June 2021 1,230 7 View

Is there another measurement method than HRM to measure velopharyngeal pressure ?

High resolution manometry (HRM) is used to measure pharyngeal and oesophageal pressure during specific tasks. Most often during swallowing. It consits in a catheter with 36 sensors spaced 1cm...

20 May 2021 1,652 0 View

Is it the right method to transform path coefficients into correlations before meta-analysis?

We all know it is possible to get path coefficients from correlation matrix (e.g., PATH ANALYSIS I: INTRODUCTION (ecu.edu) ). Using a simple solver on a spreadsheet, it is therefore possible to...

23 February 2021 8,690 3 View

Why does my protein refolded to beta sheet during thermal denaturation analysis?

Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...

06 August 2024 1,989 3 View

The Optimal Experimental Approach for Gene Function Analysis?

What is the most suitable experimental setting to understand the consequences of a particular gene: (1) targeted degradation of the specific transcription factor (TF) of the gene, followed by...

04 August 2024 6,265 1 View

Transfection in HEK293T cells?

Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...

31 July 2024 9,892 4 View

How to retain the a GFP tagged gene expression in stable cell line?

Hello , I established a stable cell line expressing GFP tagged to a centrosomal gene having G418 drug selection marker. I validated the stable line by IFA and Western blotting, results are fine....

29 July 2024 5,007 0 View

What is the relationship between protein structure and N or C terminal tagging choosing?

I want to do 2,3-butanediol dehydrogenase(BDH) enzyme purification to confirm its activity for 2,3-butanediol. Before that, I need to confirm which N or C terminal tagging is better for enzyme...

28 July 2024 366 3 View

Why cannot i find my protein on cell surface after antibiotic selection of expressing plasmid?

I cannot confirm cell surface protein expression by flow cytometry even after transfection and antibiotic selection of cells. Does it take long for proteins to express on the cell surface? the...

28 July 2024 3,178 3 View

EDC carbodiimide and NHS easter 's half life among different pH?

It has been long known that EDC carbodiimide and NHS easter's half life agains hydrolysis are highly related to pH. However, very surprisingly, not yet found a paper giving a table/chart of their...

25 July 2024 8,738 0 View

Does post-translational protein modification cause devisions on observed pI verses calculated pI?

In running two-dimensional gel electrophoresis on bacterial protein, some spots that appear to match a protein sequence have a significantly more acidic isoelectric point than the calculated pI....

24 July 2024 8,076 3 View

What is the function of fusion tag GAMGSGIQRPTST?

I found that several expression constructs of published protein structures containing an N-teiminal fusion tag GAMGSGIQRPTST. What's the founction of this fusion tag?

23 July 2024 1,353 4 View

How should we increase the quality of RNA extraction?

I’m having difficulty achieving high RNA integrity in my samples. Although the 260/280 and 260/230 ratios are satisfactory after RNA extraction, the RNA samples show signs of degradation when...

22 July 2024 155 4 View