Is it the right method to transform path coefficients into correlations before meta-analysis?

We all know it is possible to get path coefficients from correlation matrix (e.g., PATH ANALYSIS I: INTRODUCTION (ecu.edu) ). Using a simple solver on a spreadsheet, it is therefore possible to...

23 February 2021 8,366 3 View

How can one use PSO to find finite non continous parameters (e.g. enums) ?

Hi, I am making an implementation of PSO for deep neural networks training. For that, I would like the PSO to find the best activation functions as well. However, unlike weights, I use a set of...

12 November 2020 1,506 3 View

Interactions in non-experimental data (agriculture) ?

24 September 2020 2,954 5 View

How to identify trees that have not recovered but are not yet dead after transplanting in urban environnement ?

Are there reliable and easy-to-measure criteria to identify trees that have not recovered after transplanting, but are not yet dead? In particular, I seek to know the reliability of criteria such...

31 August 2020 728 2 View

Any advice for an econometric model with panel data and a lagged variable ?

Hello, I am studying the nutrition status of households using a panel data survey of South-African Households. I would like to study the changes in the nutritional status of households between...

23 April 2020 5,017 6 View

How can I get the required sample sizes for PLS-SEM using G*Power?

I have tried to compute sample size with G*Power but I cannot get the results indicated in the table 1.7 of the book by Hair et al. A Primer on PLS-SEM. Have you tried to check this ? Have you get...

24 March 2019 5,596 1 View

Who knows a botanist in Mexico who could help me by collecting Mexican species of Gentiana?

Because of time limitation, I will not be able to visit Mexico in summer 2019. Yet, I would need a few species from there for some phylogenetic work by autumn 2019. These species are: G....

02 March 2019 9,199 2 View

Rate of oxidation of various materials?

Dear colleagues, Would any of you guide me to literature papers or websites which compiled the temperature dependent (T>1000K) oxidation rates of various materials (metals, ceramiques,...

23 January 2019 1,746 9 View

Does anybody have experience in the in vitro generation of IFNy-secreting NK-cells from murine progenitors (LSK) ?

28 May 2018 7,911 3 View

What is the simplest and cheapest method for coating glass with a thin 100 micron uniform layer of epoxy or silicone resin?

I want to make a uniform thin coat of resin /pigment on 5*100 cm glass sheets. The glass could be cut before or after the coating is applied. The coating should be very even in thickness. Any...

10 February 2018 2,569 5 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

LEDs on Vis Light photo degradation of MB water sol via N doped TiO2 in a mini flume?

Good day to all, I have no experience with light emitting diodes. I am building a glass mini flume to demonstrate the photo degradation of Methylene Blue in water running over self cleaning...

26 February 2021 4,026 5 View

Why do a weight decrease for thermal degradation in nitrogen gas condition?

Hi all The question is related to TGA(Thermogravimetric analysis). Thermal degradation easily understood but why do samples weight decrease? altough just binding is broked, the weight of...

26 February 2021 1,100 5 View

How to replicate the well conditions of a crystal hit with 20%(w/v) isopropanol?

I had a crystal hit in a well with 0.1M HEPEs pH 7.5, 10%(w/v) PEG 4000, and 20%(w/v) isopropanol. Why is isopropanol in w/v here if it's just solvent? How do I recreate this well condition?

25 February 2021 9,761 1 View

Problem with ligating 2.8 kb insert into 8.5 kb plasmid?

I am trying to clone 2 copies of the same mammalian gene into the pSF-CMV-Ub-Puro Ascl (contains HindIII, KpnI and NheI cut sites) plasmid. This is what the final product is supposed to look like...

24 February 2021 6,310 3 View