I used cy3 labeled aptamers to treat the mice with tumors. I will examine the frozen slides with immunofluorescence. I was wondering has anybody stored fluorescence labeled frozen tissue slides at -20C for 2-3 weeks prior to staining?
Hi Pinar,
we work for years and years with Cy3 conjugated probes and never had any problems storing samples at -20C. You might want to ensure that you use proper mounting medium. I use with Prolong gold and Vecta Shield with great success.
They should be fine, as long as they are in the dark. And just as Ludger suggests, I have had good success with Vecta Shield. Good luck!
Hi Pinar,
I agree with Ludger and Rosa. Store the samples in dark and Use VECTASHIELD Antifade Mounting Medium for a minimal loss in fluorescence. Good Luck !!
Hello
i Agree with all , Store the samples in dark & use vectashield
Good Luck
http://mousepheno.ucsd.edu/tunelontissue.shtml#
https://www.rndsystems.com/resources/protocols/fluorescence-protocol-10
http://www.bosterbio.com/media/pdf/SA1076_DS.pdf
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