When available, are you planning to receive SARS-CoV-2/COVID-19 immunization? What do you most fear about it?

If possible, let us know about the which vaccine would be available in your geographic location.

06 December 2020 3,527 23 View

I'm looking for a protocol for doing fluoresence microscopy to see the mitochondria shape/AR, on PBMCs from human blood?

I'm looking for a protocol for doing fluoresence microscopy to see the mitochondria shape/AR, on PBMCs from human blood?

05 November 2020 8,841 3 View

Checking for overfitting in niche models?

In niche models, overfitting has been traditionally assessed by large difference between training and test AUC or other model performance metrics. Are there other methods to assess model...

07 October 2020 9,060 3 View

How do we balance the argument on the effect of hydroxychloroquine in Covid-19 prophylaxis and treatment?

Hydroxyl-chloroquine has been listed as a potential drug in the management of Covid-19. The effect of this drug in Covid-19 prophylaxis and management is trending on social media. The Doctors...

29 July 2020 2,552 4 View

Positive value in NCT real-time PCR?

Hi I will be happy for your help. I'm doing real-time PCR with two primers called MT-TL1 and 18S, for mtDNA and houskeeping gene, and I got positive control on both NCT. I tried also different...

07 March 2020 3,953 4 View

Can anyone help with a detailed assay procedure for the spectrophotometric measurement of protein carbonyls in tissues?

Protein carbonyls are important compounds to measure progression of oxidative stress in tissues. Available articles online do not have details about how to do final estimation/calculation of the...

06 February 2020 2,897 8 View

What significance is the following?

What is the significance of the following solutions being used as rodent"s organ fixatives, between normal saline, citrate buffer and sucrose?

09 July 2019 5,580 1 View

Suction pump of the Rotary evaporator?

Can someone explain why the suction pump of the rotary evaporator sucks solvent from the condensation chamber, also pumping out engine oil when it's being switched off and perhaps the solution to...

19 June 2019 3,530 13 View

Where can i purchase tail cuff device?

Anyone with the idea of where to buy tail cuff device for measuring the blood pressure of rats. Appreciation in anticipation. Fasan

10 April 2019 2,700 2 View

Defat before phenolic rich fraction extraction?

06 April 2019 2,866 8 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Errors in DNA sequences?

I am trying to better understand the scope of DNA replication and sequencing errors, e.g. 1. I have seen similar error rates of 10e4 to 10e5 for cell & instrumental DNA replication,...

24 February 2021 4,397 3 View

What might be the reason for obtaining two bands in plant genomic DNA extraction?

I got a thin band and a thick band i guess it would be the genomic DNA and the 260/280 ratio is 3.

21 February 2021 6,523 6 View

Protocol for membrane extraction E. coli?

Hello! I am interested in determining whether a poly-unsaturated fatty acid I have engineered E. coli to produce is being incorporated into the membrane of the cells or if it is simply a free...

21 February 2021 7,447 2 View

I can detect my protein but the encoding DNA sequence has stop codons within. Should I ignore the stop codons?

I constructed a synthetic DNA library (scFv, VH-VL orientation) with a 3' reporter and 8x histag. I cloned and expressed this gene following which I performed an ELISA. The ELISA results suggested...

19 February 2021 7,006 5 View

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17 February 2021 4,217 3 View