you can load the whole sample on gel, then you can cut the region of gel that contain the desired band. finally, to purify the DNA you can use gel extraction procedure.

you can load the whole sample on gel, then you can cut the region of gel that contain the desired band. finally, to purify the DNA you can use gel extraction procedure.

Thank YOU @khaled Musa Saleh

But if the size of plasmid is larger than 40kb.........then this process can't work.

Syed,

Look at similar discussion https://www.researchgate.net/post/How_to_separate_plasmid_and_genomic_DNA

and read the relevant article:

Frerix A, Geilenkirchen P, Müller M, Kula MR, Hubbuch Separation of genomic DNA, RNA, and open circular plasmid DNA from supercoiled plasmid DNA by combining denaturation, selective renaturation and aqueous two-phase extraction. J.Biotechnol Bioeng. 2007 Jan 1;96(1):57-66.

Regards

Alex

Thank YOU @ Alex Ignatov.