Genome-wide Prediction of promoter is a difficult task as they are not conserved and presence of multiple promoters and presence of promoters in the introns complecate this task. Actually we scanned the entire rice genome for promoters using Softberry promoter prediction tool and used those promoters which lie upstream to the genes for the work. It may be easy to predict promoters if you can extract 3000 nucleotides upstream regions of all protein coding genes.