How I can find signature (unique) sequences in 16s rRNA from different species of Pseudomonas?

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Abhijeet Singh

Here you can find primers and complete protocol for 16s sequencing of Pseudomonas

Wagner J, Short K, Catto-Smith AG, Cameron DJS, Bishop RF, et al. (2008) Identification and Characterisation of Pseudomonas 16S Ribosomal DNA from Ileal Biopsies of Children with Crohn’s Disease. PLoS ONE 3(10): e3578. doi:10.1371/journal.pone.0003578

Sai Shiva Krishna Prasad Vurukonda

Once you have the 16s rRNA gene sequence, there are many online websites for specific primer design. For instance try https://wheat.pw.usda.gov/demos/BatchPrimer3/

Abduladim Abobaker Hmmier

Find the attached pdf file. I guess it matches your research work.