we have few B lymphocytes and we would like to study cells individually
Hello
This a three DNA extraction methods from a single lymphocyte or blastomere and compared amplification efficiencies DNA extraction by :
(A) lysis in distilled water with freeze-thawing and boiling;
(B) two-step lysis involving potassium hydroxide and dithiothreitol;
and (C) the spanning protocol, using N-lauroylsarcosine
Good Luck
http://www.gene-quantification.de/tsuchiya-singlecell-2005.pdf
http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0041361
Hi Innas
You can have a look on this spanning protocol. Hope it will be useful for your reserch
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3455149/
there are several ways, if you do one or two amplifications you can just do PCR with one cell in tube, If you are looking gor genome amplification - ther are Lyse-and-Go protocols
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