Hello

This a three DNA extraction methods from a single lymphocyte or blastomere and compared amplification efficiencies DNA extraction by :

(A) lysis in distilled water with freeze-thawing and boiling;

(B) two-step lysis involving potassium hydroxide and dithiothreitol;

and (C) the spanning protocol, using N-lauroylsarcosine

Good Luck

http://www.gene-quantification.de/tsuchiya-singlecell-2005.pdf

http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0041361

Thank you Dr. Alkarim. I highly appreciate it (Y). 

Hi Innas

You can have a look on this spanning protocol. Hope it will be useful for your reserch

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3455149/

there are several ways, if you do one or two amplifications you can just do PCR with one cell in tube, If you are looking gor genome amplification - ther are Lyse-and-Go protocols