Inas Alhudiri

4 Questions 3 Answers 0 Followers

Questions related from Inas Alhudiri

Pcr purification without using kits or columns?

is there any protocol to purify PCR products without kit or column, I have small amount of PCR and I dont want to lose it, can I use phenol/chloroform and etoh precipitation for this purpose? thanks

12 December 2018 7,134 3 View

How I can find signature (unique) sequences in 16s rRNA from different species of Pseudomonas?

I am working on Pseudomonas. savastanoi and would like to find the unique sequence of its 16s rRNA to design specific primers. I had a look at Ribosomal database project programs but could not...

03 March 2018 1,141 3 View

What is the best media/solution for preservation or transfer of samples from the gingiva for bacterial identification and PCR? is TE buffer enough?

I have read many researchers used VGMA III and Stuart media but we don't have these media in the lab right now and would like to process my samples quickly. would appreciate your advice regarding...

03 March 2018 1,640 3 View

How can I extract DNA from a single B lymphocyte or any other cell?

we have few B lymphocytes and we would like to study cells individually

03 March 2016 8,582 4 View