In your gel you have to see 2 clear band (28s & 18s)

for more details look at link below. Just consider that gel shows quality not quantity.

https://www.researchgate.net/post/Can_anyone_help_me_with_gel_electrophoresis_of_total_RNA

Good luck

Hello Laura Giuseppina Di Pasqua ,

After denaturing the RNA with quick heating and snap cooling, you can run total RNA on 1% Agarose gel in TAE or TBE. Though native RNA can also be loaded with proper loading buffer containing ficoll, xylene cyanol, bromophenol blue etc along with a proper RNA ladder.

Clear distinction of 28S and 18S RNA bands can be observed after staining the gel with EtBr or SYBR.

Hope it helps.

Thank You so much To You all! I'll try using your advises!!!

You can make an ordinary electrovor in agaron gel as well as with DNA. If you see 2 strips corresponding to 2 subunits of rRNA, then your RNA is good. On my electrophoresis 4 ss RNA ( on left) and 2 ds RNA (on right). It is believed that RNA can be destroyed in the process of electrophoresis, in fact there is no.