Hi, I'm having problems growing the cell line PHM1-41 from myometrium. For it growth I'm using culture medium DMEM with its supplements (antibiotic and FBS). However they are growing very slow or they aren't. How do you usually grow it?
Hello Miguel,
Your difficulty growing the PHM1-41 cell line from myometrium is perfectly understandable. Growing cell lines can be challenging, and it's great to see you're trying different approaches. Here's how I usually grow this cell line:
These are the steps that I follow to grow the PHM1-41 cell line. However, cell lines are known to be temperamental, and sometimes, you may need to try different cultural conditions to achieve optimal growth. If these steps don't work, you may need to try another culture medium or change the FBS source.
I hope this helps. Good luck with your experiments!
Yours sincerely,
Edgar M Cambaza
Thanks for your answer Dr. Edgar.
Now, we are using DMEM medium, supplement with 10 % FBS( with ambitiotic and antimicotic), but they have not been able to grow. We got it when they were in pass 13, from ATCC; however, right now they are in the pass 22, thus, they aren't very old.
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