I want to enlist all the metabolites found in a particular plant. I have a very limited knowledge on chemical processes like chromatography. I wanted to know whether an HPLC can isolate particular secondary metabolite molecules from a mixture. And if it can I want to know the principle behind it. Any material on the basics of HPLC is welcomed. Thanks in advance.
HPLC is probably not suitable and first you need to define what kind of component you want to purify.
Looking for the theory of preparative HPLC, just Google and you will find for example
https://www.agilent.com/cs/library/applications/5989-5948EN.pdf
Looking for examples ?
https://www.agilent.com/cs/library/applications/5989-5948EN.pdf
Good luck
Depending on your method and the kind of column, it can be different. Aditionally, the quality of your detector, lamp and optic, also important to your purification. Somtimes, you havd to seperate one time and collect the droplet, and by reinjection to HPLC, maybe new method, you can purify nicely.
following link is a very useful book:
https://www.crcpress.com/High-Performance-Liquid-Chromatography-in-Phytochemical-Analysis/Waksmundzka-Hajnos-Sherma/p/book/9781420092608
Dear Md Fahmid,
The use of an HPLC system, including perhaps a UV/Visible detector (depending on the nature of your secondary metabolites), plus a fraction collector should be very useful.
Kind regards,
Ade Kujore
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