It’s mean that your sample (protein sequence) has 99% identity to E.coli strain K12 proteome and 100% identity to family Enterobacteriaceae compared to Genbank database.

On the other hand, you should also consider about several things:

a. number of your protein sequence identity (........sequence /.......sequence)

b. e-value and gap

c. source of your isolate

Hope it’s helpful.

@miral

the protein has been isolated from e. coli k12 proteome. uniprot kb database

@effendi

see thats my question. enterobacteriaceae is an entire family of bacteria right? so how does a protein hit 100% identity to a whole family? and e. coli belongs to that family so isnt that supposed to be a 100% identity too?

Enterobacteriaceae is a large family of gram negative bacteria. Sometimes, referred as enteric bacteria for example: Salmonella, Shigella, E.coli, Serratia, Yersinia, Klebsiella and many others.

In your case, try to find the information of your protein whether is it specific or common protein that can find in all enterobacteriaceae family.

If your protein is not specific in E.coli K12 proteome, I think it’s ok if 100% identity with whole family of Enterobacteriacea. That’s mean your protein also can be found in other genera of enteric bacteria.

So far, I think that your target identification is true enough. Not always 100% identity to enterobacteriaceae should be 100% identity to E.coli K12 proteome too. That’s why you should also see the gap sequence and e-value.

thanks :)

You’re welcome.