Md Fahmid Hossain Bhuiyan

8 Questions 14 Answers 0 Followers

Questions related from Md Fahmid Hossain Bhuiyan

Can anyone suggest me any dyes for observing plant cells under microscope?

I am preparing callus suspension culture of plants. So I am hoping to find dyes (not fluorophores) which are somewhat specific for plant cells.

07 July 2019 4,717 5 View

How to obtain the 5'-UTR region sequence of a particular eukaryotic gene (In silico)?

I need to obtain a long stretch (specific amount) of the sequence from the upstream (Upstream of the start codon) of a particular gene.

03 March 2019 2,986 5 View

How to design a gene specific primer which does not share any sequence identity with the genome sequence of any unintended organism?

I want to perform a transformation process where I want to transfer a plant gene into a another plant. I want to design a specific primer for that gene which will not share any sequence identity...

12 December 2018 952 13 View

How to find structures of ligands for proteins?

I am looking for some ligands which bind to particular parts of a protein. Here by ligands, I am pointing to a database of all known ligand structures. Is there anything available like that?...

09 September 2018 2,877 4 View

A certain protein shows 99% identity with E. coli and 100% identity with enterobacteriaceae. what does that mean?

i know that E. coli belongs to family enterobacteriaceae and the protein under consideration belongs to the E. coli K12 proteome.

06 June 2018 9,001 5 View

What do hyphens in between primer sequences for PCR mean?

I found a primer sequence for a particular gene from a publication. The sequence is- 5'-ATGGCAGTAGTAGCAG-CTCC-3' (forward) 5'-TCAGGTGTCT-AAGTTCAGAGATTC-3'(reverse) What does the hyphens...

05 May 2018 7,120 1 View

How good is HPLC for the purification of a particular secondary metabolite?

I want to enlist all the metabolites found in a particular plant. I have a very limited knowledge on chemical processes like chromatography. I wanted to know whether an HPLC can isolate particular...

02 February 2018 1,071 4 View

How can I construct the structure of a protein which have multiple sub-units?

I want to construct the structure of a hetero dimer protein. How can I do that?

01 January 2018 7,812 6 View