Is it just saline or for example CSF or is it combination of DMSO and CSF for instance?
Anyone had a problem regarding the infusion of DEX-BSA?
25 February 2020 3,703 1 View
For a Taq polymerase reaction, let's say that I have a primer A (which I cannot prevent from being extensible, it has a natural 3' end) which can hybridize to a known point on a synthetic DNA...
27 January 2020 9,085 3 View
I've been reading the Johnson & Goody translation of "Die Kinetik der Invertinwirkung" and I can't find the part where authors (M&M) actually claim that [E]0
15 March 2017 3,836 4 View
I have multiple parameters and want to examine their effect on the response variable, Y. The experimental design is as follows. There are 3 factors. Two of them: A1 and A2, have two levels (coded...
09 December 2016 4,440 16 View
In modeling composite plates in a Nastran/Patran program using shell elements, results in the output file are for the mid-plane of each layer of composite. Does anybody know if it can be possible...
24 August 2014 1,725 6 View
I need protocol, if there is one, to separate BSA from compound DEX-BSA. I attached a chemical structure of DEX-BSA?? Any idea, protocol will be very helpfull.
21 August 2014 4,896 1 View
Although I am fully aware that different primer designing software are using different algorithms in their work, still they should give the similar primer pair combinations or not? Also, when some...
31 July 2014 2,648 1 View
I run real time sybr green pcr (rat hippocampus) and I came accros a primer dimer situation. I use 42.5 nM primers in final concentration. I am getting PDs in whole range of concentrations, from...
07 July 2014 3,890 3 View
Advantages/disadvantages, troubleshooting when doing real time PCR?
27 April 2014 8,274 9 View
What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...
03 March 2021 1,401 3 View
We have HUVEC bought from Invitrogen. Cat no -C-003-5c. while first plating the cells we have bought the protocol suggests not to centrifuge the cell with media to remove the DMSO. Should we...
02 March 2021 3,713 2 View
Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...
02 March 2021 3,024 2 View
I am at the beginner level in drug design and drug discovery, and I am interested to learn more about this field. Which books are highly recommended to start from and gives me all essential...
02 March 2021 8,437 2 View
I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...
01 March 2021 8,544 1 View
After I cryofreeze the cell do I need to remove the DMSO for culturing the cell for next passages .
28 February 2021 7,738 3 View
What could be the reason of moisture at the sides of Vial when I am preparing my polmyer+DMSO solution for membrane fabrication? What etiquettes I am possibly missing here?
28 February 2021 6,516 5 View
The antimicrobial effect of local anesthetics has been known for more than 50 years, but it still seems to have an unclear mechanism of action. which bacteria? which concentration? etc etc. ....
27 February 2021 4,450 2 View
Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...
25 February 2021 3,443 4 View
My purpose is to recognize the volatility clustering property without statistical tests such as The Lagrange Multiplier (LM) test.
24 February 2021 4,150 3 View