I've found that one of my drugs (peptide) causing shrinkage of nucleus and microtubule destabilization. I have also found that the drug is causing apoptosis of cell via p53 and p21 pathway etc. The live cell imaging shows shrinkage of nucleus more than 1/2 w.r.t control cells (without treatment). I want to know whether the effect is common bcz of cells are dying, hence cell cytoplasm to nuclear ration getting decreased or any other reasons...
Your peptides activate PLA2 and likely leads to caspases independent nuclear lamin cleavage.
See
http://jcb.rupress.org/content/163/6/1185.2
http://jcb.rupress.org/content/163/6/1219.full.pdf
Thanks Goodwin.I hv gone thru the JCB article it was shown tht the hypoxic condition activates PLA2 and further caspase independent cell death , and it is caspase independent apoptosis. The mechanism for hypoxic cell death is based on PLA2 activation.If my compound is activating PLA2 then can we say that it is causing caspase independent hypoxic cell apoptosis?
You'll have to check
1.PLA2 activation
2.Check similar nuclear shrinkage occurs or not, when you put the peptide+zVAD-fmk
3.Check the levels of HIF1a changes with or without peptide
Then you'll be able to say as you wished for.
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