How to interpolate from a time-series database ?

Hi, I am having trouble with a problem, in the field of Optimal Control and the generation of optimal time-series. Let's consider a system, whose dynamics are represented by dx/dt =...

24 February 2021 9,105 2 View

Are international conferences a dying species?

In our discipline (chemistry), annual meetings of the American Chemical Society (ACS) used to be mega-events with up to 20,000 participants. The current one will be virtual, and the organizers...

06 January 2021 1,882 5 View

How to avoid rejection of your research paper?

Despite certain disadvantages, peer-review is generally accepted as a quality control of manuscripts submitted to scientific journals. The higher the rank of the journal, the more often...

03 December 2020 3,235 17 View

How important are citations? Are they overrated?

Citations of research papers are the topic of various threads on this platform. Quite often researchers ask how to increase the number of citations. I did my first 30 years of chemical research in...

01 December 2020 6,173 94 View

How to find the number cells in each LSTM hidden layer from python code?

LSTM has two hidden layers, I want to know the number of cells in each hidden layer.

30 November 2020 3,481 4 View

How can we sell chemistry to the public?

Chemistry is frequently termed the "central science". It underlies the foundation of modern civilization (drugs, plastics, textiles, dyes, fertilizers etc.), but the public perception of chemistry...

30 November 2020 1,113 15 View

How can chemistry help fighting COVID-19?

Chemistry is frequently termed the "central science". As such, chemistry is expected to play a major role in fighting the coronavirus. For example, hand sanitizers and drugs are all based on...

30 November 2020 4,983 17 View

What was your most exciting research achievement?

As dedicated researchers we all try to contribute to the advancement of our discipline. Often the work can be routine, but occasionally there are some real highlights. Can you please share your...

23 November 2020 4,150 20 View

Is vertical farming the future of agriculture?

Please provide your opinion on vertical farming. Is it the future of agriculture? If yes, where is it most promising (areas, countries cities etc.)?

17 November 2020 3,934 97 View

Is peer-review censorship or necessary evil?

Most researchers have made individual experiences with publishing in peer-reviewed journals. What is your opinion about the peer-reviewing process in general? In your opinion, is it "censorship",...

28 October 2020 3,631 97 View

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02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

01 March 2021 210 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Expression cloning by using cDNA,do I need to modify the cDNA first?

I am going to have a expression cloning of mammalian gene by using shuttle plasmid to transforming the E.coli However I don't know I should only inserting the Coding sequence ,or I can...

28 February 2021 5,440 3 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View