Does ozone layer present in uppermost layer of earth atmosphere?oxygen is below it ? However ozone molecular weight greater than oxygen? Am i Right?

Biotransformation

10 November 2017 9,220 3 View

What is possibility of detection by ELISA?

Hi, In one of bizarre finding, I got no binding in ELISA for one set of mAb expressed transiently, however positive and negative including Test2 sample gave expected results. Further when...

10 November 2017 7,117 2 View

Can anyone help me increasing transient expression (Lipid mediated transfection) of protein in CHO cells?

I want to know the ways by which i can increase expression and transient transfection efficiency of CHO cells. Please let me know any efficient additives, Temperature condition, Transfection Kits...

10 November 2017 6,080 6 View

Detection of Agonist, Partial Agonist and Antagonist antibody type?

Hi All, I am dealing with the problem of having mixed nature of monoclonal antibody in the system that is in terms of its activity of showing efficacy. I would like to know if someone could help...

08 September 2016 9,772 3 View

Is it possible that if cell bank were made from suppose X medium had 98% Viability however on revival on same media not supporting growth?

My cloned cells are not supporting growth using the same media. It was kept in cryopreserved state in cell banks. Can any one help me to get an idea like how its possible? pH is maintained thus...

06 July 2016 1,745 2 View

Best media for CHO cell lines?

Can anyone help me to get which media i should use to get maximum biomass for CHO cell lines?

06 July 2016 4,050 1 View

What is the role of nitrogen source for growth of bacteria in media?

I am working on Bioremediation.

01 February 2016 7,007 2 View

What are the different methods to preserve cells for long time & why used different method to preserve cell ?

I am working on Bioremediation

31 December 2015 8,076 1 View

How to make standard graph of any reagent or molecule or compound ?

I am working on Bioremediation.

31 December 2015 3,366 2 View

If microorganism present in media and then autoclave that media. Is there any change in the media composition due to denatured protein of bacteria?

I am working on bioremediation.

31 December 2015 3,967 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

Why almost every element would be metallized in sufficiently high pressure and low temperature?

(This statement is from wikipedia, BTW). What is so special about metallic bond that is limiting high-P low-T state of a matter (as long as individual atoms exist, not white dwarf of neutron star...

02 March 2021 3,309 2 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

I have designed reflectarray element and feeding it though Horn Antenna on HFSS. After full wave simulation, I am not getting desired results?

I have designed reflectarray element to generate OAM beams at 5.8GHz and feeding it though Horn Antenna on HFSS. After full wave simulation, i am not getting desired results i.e Radiation pattern...

01 March 2021 9,701 5 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Any research papers for extraction of nutrient deprivation on macroalgae? Analysed by HPLC-UV and GC/MS. Possibly from macroalgae genus Rhizoclonium?

I am doing my thesis on the nutrient deprivation on the macroalgae Rhizoclonium sp. I will be analysing the levels over a period of roughly 5 days by HPLC-UV and GC/MS. I will be using seawater as...

28 February 2021 6,477 1 View

Microstrip antenna design tutorial of HFSS 3D layout module ?

I have designed a 24 GHz rectangular antenna on HFSS 3D model and got desired results while desing the same antenna with same dimensions I am getting resonance frequency at 27 GHz I am...

28 February 2021 4,426 2 View