Selecting particular columns from large datasets (csv) using R Studio?

So I have large number of csv files (100+) in a directory with same structure and column names. The data is in time series and there are approx 30 columns/file. However i only want to aggregate...

08 February 2021 245 5 View

How much pre-PCR dilution of mixed-template DNA extracted from pollen (honey) is appropriate for Metabarcoding?

I'm working on pollen DNA metabarcoding to identify the floral composition of honey. How much pre-PCR dilution of mixed-template DNA extracted from pollen (honey) is appropriate for the...

28 December 2020 9,132 1 View

How to add two vertical and parallel Fresnel reflection coefficients to retrieve overall reflection coefficient for a right hand circular wave?

I have a problem in which a right hand circular polarized wave (GPS wave) encounters an ideal smooth plane obliquely, and then is reflected off that. To solve this kind of problems, the wave is...

28 December 2020 2,271 3 View

Which is better to focus on teaching or research for a good academic life and Why?

Which is better to focus on teaching or research for For a good academic life and Why?

26 December 2020 5,737 9 View

Is ag paste suitable for back contact on silicon wafer? or i should deposit al on the back surface using pvd or plasma sputtering?

Fabricating silicon solar cell

22 December 2020 4,906 7 View

Which renewable source is the best ?

wind hydro solar etc.

21 December 2020 6,360 16 View

Why is light pure energy?

light is pure because the light is the simplest electromagnetic construct but the energy of any object is also of the same kind.

20 December 2020 207 4 View

How to handle Missing Values for Skipped Questions (Systematic Missing) in R ?

10 December 2020 435 4 View

What is the best industrial emulsifier to make silicon oil in water emulsion?

I need to make long-lasting stable emulsions of 6 wt. % percent silicon oil in DI water. The previousely made emulsions using Tween and Span Emulsifiers did not remain stable after a week.

31 October 2020 3,568 3 View

Carbon Emission calculation factors ?

I am thinking about writing a paper on carbon emissions based on electricity consumption data from some buildings. From a research perspective, which factors should i specially focus while...

09 October 2020 4,142 3 View

Does the pressure(-600 mmHg) affects pH?

When I conducted the degassing experiment using decompression under anaerobic condition(and I used ferrous based chelating agent), the oxidized ferric were observerd. So, could you tell me the...

03 March 2021 4,892 5 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Genome-wide gene identification protocol ?

Is there any book chapter/book, webpage or research article available to understand genome-wide gene identification?

28 February 2021 8,095 1 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

Which database would give me this list of chemical components?

Dear colleagues: I would like to know if anyone knows of a database that gives me the parameters pH, K, K, Mg, P, C, N, Zn, Cu, Ni and Cd of common foods. I find a lot of information of proteins,...

26 February 2021 5,525 3 View

RNA-seq DEGs downstream analysis and reporting in literature?

I used EdgeR to get the list of DEGs (differentially expressed genes) from my liver RNA-seq experiment. I have done the gene overrepresentation and GSEA based pathway analysis. I am currently...

23 February 2021 8,969 1 View

Why is NH4OH added in heavy metals limit test?

The limit test I'm referring to is USP method II. In this procedure you need to dropwise add 6N of NH4OH to the sample until it the pH is alkali. After that you need to dilute and add HOAc until...

23 February 2021 2,049 3 View

Could you please suggests a tutorial or paper or any other materials to learn multiple gene expression in TCGA and GTEx databases?

I would like to analyze multiple gene expressions in normal and cancer tissue by using the data in the TCGA and GTEx databases. Please suggest a tutorial or paper showing step by step analysis of...

23 February 2021 9,007 3 View

Is there an ideal incubation time of RGO-modified SPCE before subsequent use, i.e., electrochemical characterization or further modification?

I notice that upon washing and air-drying of my RGO/SPCE surface and characterizing again via DPV, the current response towards ferricyanide probe significantly decreases. My worry is that the...

22 February 2021 5,324 3 View

No title

22 February 2021 9,932 11 View