How does one trace draining lymph nodes of internal tissue?

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How to reduce the time taken for simulations in NetSim?

When I run large simulations with 100s of devices it takes several hours for the simulation to complete. So if I need to run like 20 - 30 different parameter settings then it would take me several...

05 November 2020 7,812 2 View

How can I automatically run a large number of simulation in NetSim?

I want to do a multi-parameter sweep by changing the value of one parameter for each run. It is cumbersome to do it via GUI and I wanted to know how to automate this in NetSim. Thank you.

13 October 2020 5,283 3 View

I am working on gelatin based product. On HPLC gelatin chokes column. Can someone please suggest a way out to degrade gelatin?

I am analyzing gelatin based product on HPLC. But gelatin being complex molecule chokes column. This issue may get resolved, if I break gelatin into smaller molecules. Please suggest some ways to...

26 August 2020 6,895 2 View

Does a cured Person from CoVid-19 acts as a carrier ?

Viruses can not be killed. If so does a person infected from CoViD-19 doesn't acts as a carrier after his cure? That is if a person is infected with CoViD-19 and he is been cured by the medicine,...

30 March 2020 498 11 View

Can a thermoelectric material may have negative lattice thermal conductivity?

I have calculated electronic components of thermal conductivity by wiedmanfranz law. Then the lattice thermal conductivity is derived from it. But some of those values are negative. at the end of...

26 January 2020 1,507 4 View

Reference article for solubilization technology guidance map?

Dear all I read one literature in past, which discusses selection of solubilization technology based on the melting point and log P of active pharmaceutical ingredient. The faint content back of...

17 December 2019 6,848 3 View

Can we perform pair distribution function analysis using Microsoft Excel?

I am willing to get insights on how to perform pair distribution function analysis of powder diffraction data obtained for amorphous pharmaceutical. I have googled and found some software...

26 November 2019 8,763 4 View

How can we relate the binding energy measured from XPS for metals/alloys to their d-band positions?

In other words if the binding energy measured for the alloys from the XPS shift to higher energy levels due to doping, does that indicate d-band energy levels shift to more negative...

14 November 2019 6,521 2 View

How to do optimization of array antenna on CST using Genetic Algorithm?

I have made an antenna array and want to optimise using GA. Kindly tell me!!

06 November 2019 6,137 2 View

A wide band antenna is made. After some manipulation the antenna is dual band. Will the gain be same or different as that in wide band antenna?

A wide band antenna is made and after some manipulations the antenna is now working as dual band !! I want to know that whether the two Antenna will give the same gain or not ??

17 October 2019 3,383 4 View

Which fluorescent dye to use for measuring total ROS production?

Is DCFDA appropriate for measuring total ROS production, or can it only detect certain types of ROS? If I want to measure total ROS production, which dye should I use?

01 March 2021 9,447 3 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View

What is the best surface to culture cells isolated from a skin biopsy?

Hello, recently I am having some difficulties with maintaining the cell culture of primary cells isolated from a skin biopsy (normal and malignant). The coating of culture plates with Matrigel (a...

26 February 2021 6,151 2 View

Is it efficient to do liquid biopsy to investigate the gene expressions of the tumor?

To do liquid biopsy and look for specific gene expressions there are so many published papers. Thera are different protocols and procedures used to isolate circulating cell free RNA or mRNA from...

23 February 2021 7,326 5 View

In methylene blue test Why is UV absorbance reading very low as soon adsorbent is added and then increasing before reaching equilibrium?

Hello, I'm working on removal of methylene blue. 01. in the dark test very first reading is taken 15 minutes after adding adsorbent to the dye solution and that UV absorbance reading is very...

19 February 2021 472 5 View

FDC and FRC cell populations in mouse spleen?

I was trying to isolate fibroblastic reticular cells (FRC) and follicular dendritic cells from mice spleen, but kept getting poor yield. I found from literature that the frequency of FDC is around...

17 February 2021 3,506 4 View

Has anyone performed a colorimetric RT-LAMP assay using Lucigens LavaLAMP kit?

Hi there, my lab recently opted to use LavaLAMP from Lucigen instead of the Warmstart kit from NEB. However, Lucigen has yet to create a colormetric kit for RT-LAMP, and adding phenol-Red (pH...

17 February 2021 5,399 1 View

Why stop the reaction in ice for the fluorescent probe FDA (fluorescein diacetate)?

Hi, In literature I noticed that who stains haemocytes with FDA for 30 min then stop the reaction in ice while this does not apply for 15 min incubation time (or maybe authors did not write this...

08 February 2021 3,366 3 View

What is the possible cause of MSCs low growing?

Hello Everyone, We are inexperienced in culture of MSCs trying to grow a sample from BM of a mare. It is the second time that after preparation of new medium (DMEM-F12 with high glucose - 10% Hy...

07 February 2021 9,989 7 View

Why do cells die after adding MTT dye?

I was doing MTT assay, and in the last day before adding the MTT; the cells looked good (or so I think), but after aspiration of the drug and addition of MTT dye I noticed that most cells seemed...

07 February 2021 3,846 4 View