alarmins im interested in include: HMGB1 and S100 proteins amongst others
Will other cell lysate proteins mask the signal from the alarmins im interested in?
heavy use of Large Language Model will going to change the working style of organization?
23 June 2024 7,451 3 View
Can be publish any paper through research gate?
24 March 2024 10,037 3 View
Hello, I'm studying rainfall and temperature's impact on river basin water quality. Are there any indices that effectively capture both rainfall and temperature? I have only temperature and...
10 March 2024 7,224 3 View
Has anyone ever performed MASW using DAS acoustic sensing approach , as traditionally only geophone sensor are utilized. If so the answer is yes, how did they manage to acquire the datasets
29 February 2024 1,137 0 View
Resuscitation drugs become ineffective during severe metabolic acidosis ...why can't bicarbonate be a part of ACLS protocol
22 January 2024 5,099 3 View
I am looking to quantify metabolites released into cell media, especially alarmins/DAMPS and cytokines. The concentrations of these inflammatory proteins will be on the nanograms/ml or nanomolar...
03 January 2024 1,628 0 View
What are the factors affecting the measurement of electrolytic conductivity using DC and AC techniques? What are the challenges and precautions related to electrolytic conductivity measurement?...
26 June 2023 5,507 2 View
Will that be stable enough ? how can we check for its stability and efficacy?
14 June 2023 8,742 1 View
Couple stress fluid constitutive relation in vectorial form
08 June 2023 9,525 1 View
Hi there, Could anyone recommend a cheap and easy way to assay for cell death (probably apoptosis). When our gene of interest is transfected into cells, there appears to be a lot of cell death...
06 June 2023 3,347 6 View
Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...
06 August 2024 1,989 3 View
Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...
31 July 2024 9,892 4 View
Hello , I established a stable cell line expressing GFP tagged to a centrosomal gene having G418 drug selection marker. I validated the stable line by IFA and Western blotting, results are fine....
29 July 2024 5,007 0 View
I want to do 2,3-butanediol dehydrogenase(BDH) enzyme purification to confirm its activity for 2,3-butanediol. Before that, I need to confirm which N or C terminal tagging is better for enzyme...
28 July 2024 366 3 View
I cannot confirm cell surface protein expression by flow cytometry even after transfection and antibiotic selection of cells. Does it take long for proteins to express on the cell surface? the...
28 July 2024 3,178 3 View
It has been long known that EDC carbodiimide and NHS easter's half life agains hydrolysis are highly related to pH. However, very surprisingly, not yet found a paper giving a table/chart of their...
25 July 2024 8,738 0 View
In running two-dimensional gel electrophoresis on bacterial protein, some spots that appear to match a protein sequence have a significantly more acidic isoelectric point than the calculated pI....
24 July 2024 8,076 3 View
I found that several expression constructs of published protein structures containing an N-teiminal fusion tag GAMGSGIQRPTST. What's the founction of this fusion tag?
23 July 2024 1,353 4 View
hi every one I am making vector construction (for fusion proteins) and in this moment I wanna to amplification of ADAM17 prodomain with PCR. to yet, I couldn't amplified the ADAM17 prodomain with...
21 July 2024 8,660 1 View
I need to know the approximate size of my target protein for Negative-stain EM. I have an alpha-fold predicted structure of the protein. I do not see any turtorial on internet for estimating the...
21 July 2024 5,732 3 View