alarmins im interested in include: HMGB1 and S100 proteins amongst others
Will other cell lysate proteins mask the signal from the alarmins im interested in?
I need to implement matrix inversion in hardware. The size of the matrix can be anywhere from 50 *50 to 8*8. Are ready-made standard codes available or inbuilt modules available with FPGA...
15 February 2021 4,006 3 View
I am using FDD (code I used - https://uk.mathworks.com/matlabcentral/fileexchange/50988-frequency-domain-decomposition-fdd) to extract the natural frequencies and mode shapes using the...
03 January 2021 4,038 2 View
Gases in ambient air are measured in various units. Like ppm, ppb, percentages, microgtam per cubic meter etc. What are these exactly? I am new to the air field. earlier I worked with soil and...
27 November 2020 3,908 1 View
I came to know that there is no oilglands in sandalwood and it is a byproduct during transformation of sapwood to heartwood. Is it true?
04 October 2020 8,216 2 View
Different miRNA target prediction algorithms use different scores which is not comparable directly. So what is the most reliable way to get the consensus of results produced by different miRNA...
24 August 2020 9,659 4 View
RNA22, miRanda, TargetFinder, and psRNATarget are four different algorithms aiming at finding miRNA targets. Many research-work claims at using a consensus of the result produced by these...
23 August 2020 5,058 5 View
It is observed that, the cities with higher air pollution witnessed comparatively more no of deaths of COVID-19 infected patients in the world. Kindly provide your valuable inputs regarding the...
08 April 2020 4,384 30 View
Dear Taxonomist This is Satish Harde from Jalna Maharashtra India, i want to know what is the method to draw the sketch of mealybug or any insect as shown in the attached file. Thanks with...
28 March 2020 5,044 3 View
We want to see the bioinsecticidal properties of some plant extracts on Cowpea weevil (Callosobruchus maculatus) on stored Cowpea Vigna unguiculata.
13 February 2020 8,987 8 View
I am searching for a book comprising the causes, effects and solutions of global warming. It would be very helpful if anybody can suggest some book or relevant literature.
11 February 2020 1,687 4 View
I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...
01 March 2021 9,310 4 View
Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...
01 March 2021 2,622 3 View
I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...
28 February 2021 4,521 3 View
I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...
28 February 2021 7,127 2 View
I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...
28 February 2021 7,370 3 View
I had a crystal hit in a well with 0.1M HEPEs pH 7.5, 10%(w/v) PEG 4000, and 20%(w/v) isopropanol. Why is isopropanol in w/v here if it's just solvent? How do I recreate this well condition?
25 February 2021 9,761 1 View
I am trying to clone 2 copies of the same mammalian gene into the pSF-CMV-Ub-Puro Ascl (contains HindIII, KpnI and NheI cut sites) plasmid. This is what the final product is supposed to look like...
24 February 2021 6,310 3 View
Hi all, I have been doing research on biotagged LDB1 proteins in Mel cells. I purify the proteins using M280 streptavidin beads. The yield is very low so I've been trying to optimize it by...
24 February 2021 5,749 3 View
Hello, I am running a sandwich-type ELISA where I am trying to inhibit a protein-protein interaction using small molecules. For each experiment, I determine my Z' (Z-prime) to determine the...
23 February 2021 7,429 1 View
Hi all, Is there a way to extract RNA and protein from the same cell culture sample? Because we need to use the protein for gelatin zymography at later time, we are looking for a method that can...
22 February 2021 5,341 5 View