How does one extract inclusion bodies from E. coli?

More Shanice Chiang's questions See All

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Dawid Deneka Popular answer

Inclusion bodies purification is very easy and can be performed using very common reagents. You can find a lot of protocols in google.

Inclusion bodies are almost impossible to reconstitute in buffer without chaotropic agent or strong detergents. If you sonicate your cells in simple PBS or PBS/detergent inclusion bodies will surely stay in nonsoluble fraction.

Here you have a protocol for IB purification: https://labs.fhcrc.org/strong/StrongLabRefoldingProtocol.pdf

If you have any more question feel free to ask.

Dawid Deneka