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Questions related from Dawid Deneka
I have heard some rumors that the HRV-3C protease can recognize and cleave ENLYFQG peptide, which is a consensus sequence cleaved by the TEV protease. Can anyone confirm or suggest some literature...
07 July 2018 8,672 3 View
I wanted to analyze the phosphorylation of a certain mammalian protein. It is a standard procedure to add phosphatase inhibitors to extraction buffers to block the process of dephopshorylation....
03 March 2018 3,924 5 View
Dear all, I would like to ask what is the maximal achievable resolution of ion exchange chromatography in the matter of pI values. In particular I am wondering whether I will be able to separate...
10 October 2016 8,782 4 View
I am planning to prepare a 100-1500bp DNA marker using endonuclease digestion of a special plasmid. I am wondering if the presence of sticky ends can affect gel migration of generated fragments....
10 October 2013 2,078 14 View
I would like to clone a 7bkp insert into a pUC18 vector (2,7kbp). Do you know if it may be somehow problematic? I mean ligation or transformation efficiency, insert stability or any other...
03 March 2013 8,176 2 View
I would like to ask if anyone has experience with protein in silico alanine scanning algorithms. I am planning to do some research using one of the webserwers: DrugStorePPI or Robetta.Bakerlab. Do...
03 March 2013 6,266 0 View
I am trying to produce TGF-alfa using an E.coli system. The TGF-alfa gene was cloned into a pET30 vector so the construct contains (N) his-tag + s-tag + TGF_alfa (C). Those two tags with linkers...
01 January 2013 9,714 8 View
I am interested in preparing a phagemid library. I would like to know if it is very expensive (more or less) and if it needs lots of lab experience. I would be grateful for any answer!
10 October 2012 7,040 7 View
