How does one correctly multiply plasmid DNA?

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Strong signal on Southern blot hybridization: Any suggestions on how to proceed?

I need to determine the number of integration sites of the plasmid DNA into the genome of a CHO cell line. After selection, the population of zeocin resistant transfectants were cloning by single...

04 May 2014 7,488 4 View

Does anyone has a sequence of plasmid pCMV-BICEP-4?

I am seeking for sequence of plasmid DNA pCMV-BICEP-4 (5363 bp) company Sigma. I cannot find its sequence online. Maybe someone has the sequence?

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MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

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fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

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What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

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Can linear DNA be used as template for site-directed mutagenesis?

Is it possible to induce site-directed substitution mutation by quick-change method on linear dsDNA? or it has to be cloned in some vector? If yes, should it be treated with the Dpn1 enzyme...

03 March 2021 401 4 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

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Saaraj Gupta

You can try to screen random clones by checking desired components, using PCR, and then multiplying the host culture.

Michael J. Benedik

I don't understand your question sufficiently to answer. What is the problem you are trying to solve or concerned about?

Masita Mandasari

I usually multiply my plasmid by transforming them in E. coli culture then pick up several colony followed by DNA extraction. Then, I check whether I get the correct clone by either enzyme restriction or DNA sequencing.