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Questions related from Saaraj Gupta
Which plasmid should I consider for quantification using DNA ladder as concentration standard? Plasmid has to be used for restriction digestion. I need an alternate to spectrophotometric method.
12 December 2013 8,120 2 View
I need to know if there's a way I can specifically quantify (visually) his-tag fusion protein. I have checked for commercial his-tag stain reagents, but I'm looking for state-of-art method(s)....
05 May 2013 8,600 4 View
I've expressed Taq polymerase in E.coli. I have found a few reports for its purification also using sephadex columns. Next, I need to check its efficacy preferably by methods other than PCR. Can...
05 May 2013 1,021 2 View
I am looking for cheaper and still efficient state of art methods. I have checked for FACS and a few commercial kits but they are out of my budget.
03 March 2013 1,914 8 View
In their work (see attachment), Pansri et. al. has used pull-through PCR. I am unable to understand the purpose because I do not know about Pull-through PCR.
02 February 2013 7,362 1 View
