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I need to determine the number of integration sites of the plasmid DNA into the genome of a CHO cell line. After selection, the population of zeocin resistant transfectants were cloning by single...
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I am seeking for sequence of plasmid DNA pCMV-BICEP-4 (5363 bp) company Sigma. I cannot find its sequence online. Maybe someone has the sequence?
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Is there anyone who knows how to correctly multiply plasmid DNA in E.coli cells either 1) by population of plasmid or 2) by cloning and pick out single clones? If first, I collect all, include...
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