I am seeking for sequence of plasmid DNA pCMV-BICEP-4 (5363 bp) company Sigma. I cannot find its sequence online. Maybe someone has the sequence?
I was able to find the technical bulletin which contains the polylinker sequences here https://www.sigmaaldrich.com/content/dam/sigma-aldrich/docs/Sigma/Bulletin/e5905bul.pdf. I was unable to find the full vector sequence.
http://www.addgene.org/vector-database ?
I need to determine the number of integration sites of the plasmid DNA into the genome of a CHO cell line. After selection, the population of zeocin resistant transfectants were cloning by single...
04 May 2014 7,488 4 View
Is there anyone who knows how to correctly multiply plasmid DNA in E.coli cells either 1) by population of plasmid or 2) by cloning and pick out single clones? If first, I collect all, include...
09 October 2013 668 3 View
How to calculate the RMSD values for a MD simulation using MOE?
07 August 2021 0 0 View
When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.
16 June 2021 0 0 View
I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance
03 March 2021 3,568 1 View
Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...
03 March 2021 5,978 5 View
Is it possible to induce site-directed substitution mutation by quick-change method on linear dsDNA? or it has to be cloned in some vector? If yes, should it be treated with the Dpn1 enzyme...
03 March 2021 401 4 View
Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...
02 March 2021 4,569 4 View