Masita Mandasari

5 Questions 14 Answers 0 Followers

Questions related from Masita Mandasari

What causes conventional PCR product for GAPDH to be shorter than expected?

I design primer pair for house keeping gene mouse Gapdh. According to my calculation, genomic DNA template should produce 600 bp fragment while cDNA template should produce around 400 bp. I...

02 February 2015 8,657 14 View

Why I get band in my PCR negative control?

Lately I am having trouble with PCR genotyping. I keep on getting non specific amplification that have the same size with my target (400 bp). So, I know it's not primer dimer. At first I thought...

11 November 2014 4,039 15 View

Can we mix digestion buffers from different manufacturers in double digestion reaction?

I need to digest my plasmid using BamHI and SacI. Unfortunately, the enzymes came from different manufacturer, Fermentas and Wako Nippongene respectively. I checked the buffer ingredients and they...

07 July 2014 6,203 9 View

Why is my PCR band size less than expected?

My primer set was designed to yield 750 bp fragment. But, on the gel after PCR, I saw clear single band on 500 bp with very faint band around 750 bp. I purified the 500 bp fragment and did direct...

06 June 2014 4,071 12 View

What is the rationale of 16 weeks of 4-NQO treatment in mouse carcinogenesis?

I am currently doing mouse carcinogenesis experiments using 4-NQO, diluted in drinking water. Almost all papers that I read stated that they did 16 weeks administration, either topically or via...

12 December 2013 1,154 2 View