Manmeet Bhalla

3 Questions 4 Answers 0 Followers

Questions related from Manmeet Bhalla

Any idea about freezing the murine tissue samples and later using them for RNA extraction?

Hi all, Has anyone frozen the murine tissue samples (lung or spleen) in -80 and then later thawed these to go ahead with cell purification (PMNs etc) for RNA extraction? I am wondering if it...

11 November 2018 9,287 3 View

What is the effect of using higher passage number HeLa cells on entry of listeria or InlB coated beads?

I used P58 this time and saw very low entry of InlB coated beads compared to what I usually see. I was wondering if the passage number has any effect on the Met receptor expression that resulted...

05 May 2015 1,915 0 View

What is the molecular weight of mTOR expressed through pcDNA3/AU1-plasmid?

What is the molecular weight of mTOR expressed through pcDNA3/AU1-plasmid containing rat mTOR gene. I am using this plasmid and when I immunoprecipitate myc tagged mTOR with myc antibodies, I am...

08 August 2014 1,671 3 View