Hi all,
In vitro inflammasome reconstitution system is common in studies involving NLRP3, NLRP1 etc.
Some examples of murine and human NLRP3 inflammasome reconstitution is linked below:
Article Reconstruction of the Mouse Inflammasome System in HEK293T Cells
andArticle Bile Acids Control Inflammation and Metabolic Disorder throu...
(Fig.5 )I was wondering how IL1b is secreted in those experiments (both paper linked here show that IL1b can be detected in the supernatant of transfected HEK293T using ELISA). Normally IL1b secretion depends on GSDMD/pyroptosis, but 293T does not express GSDMD. So how does 293T get the pores to secrete mature IL-1b?
Thanks!
Dear Leon Hsieh
Check this interesting article.
Article Reconstruction of the Mouse Inflammasome System in HEK293T Cells
Best wishes,
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