Leon Hsieh

3 Questions 1 Answers 0 Followers

Questions related from Leon Hsieh

Can ectopic caspase-1 expression in HEK293T cause pyroptosis/cell death?

Hello all, I am designing an experiment to ectopically express caspase-1 and other components of the inflammasome to measure Il1b cleavage and release. However, I came upon this nice paper...

17 July 2019 8,678 1 View

How "deep" can you see with a confocal microscope?

Hi, I'm trying to image fluorescent labeled cells on a gel. How deep am I able to see with a confocal microscopy? What's the maximum depth of confocal microscope? I would love to hear some...

12 November 2015 4,137 4 View

How does IL-1b get secreted in in vitro inflammasome reconstitution systems?

Hi all, In vitro inflammasome reconstitution system is common in studies involving NLRP3, NLRP1 etc. Some examples of murine and human NLRP3 inflammasome reconstitution is linked...

01 January 1970 6,941 1 View