What is the size of band in the well marked by yellow arrow?

I run this gel(1%) and expecting a band of 730bp, but I can't figure out the size of band in the well marked by yellow arrow... so is this the band that I am expecting?

07 August 2018 7,870 2 View

Which 16S reference sequence should I use for Azotobacter chroococcum?

I want to confirm the Azotobacter chroococcum by sequencing it with normal 16s primers, so is there any general sequence available to be used as a reference sequence?

07 August 2018 4,849 0 View

Why is smear on gel in my pcr of C-DNA samples?

I am getting smear in the gel of my pcr of CDNA samples, and to surprice me, I am getting band in GAPDH +ve control but not in my CDNA samples... Also I incorporated 2 plasmids as +ve controls,...

11 December 2017 710 2 View

Can anyone help me about multiple bands in my Western blot?

I did Western blot of samples from cotton root tissues from same event, but of different time spans (took samples after 1 month interval for 4 months), and I got difference in banding...

06 July 2014 9,384 7 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

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02 March 2021 3,060 3 View

Should we change media after1st 6hrs after plating HUVEC bought from INVITROGEN?it's not recomended by invitrogen.Will DMSO present in stock matter?

We have HUVEC bought from Invitrogen. Cat no -C-003-5c. while first plating the cells we have bought the protocol suggests not to centrifuge the cell with media to remove the DMSO. Should we...

02 March 2021 3,713 2 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View

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28 February 2021 9,936 2 View

Benefits of the 24 welled plate?

We have always used the 96 welled plate, the 48 welled plate but apparently there is much evidence on the 24 welled plate and above all its benefits.It states it is the same you would use on ELISA...

28 February 2021 1,149 1 View

Can bacterial suspension be pipetted directly onto petri dish and agar poured onto it?

Hi, recently I encountered a protocol for plating which was not the usual method I have been practicing (pour LB agar onto plate, allow it to solidify, pipette bacterial suspension onto plate,...

28 February 2021 7,292 3 View

When is it unnecessary to change organisational culture?

As a former management practitioner, I recall a generally accepted view that changing organisational culture is difficult, so you should only attempt it if the organisational change that you are...

27 February 2021 2,595 8 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

What is the best surface to culture cells isolated from a skin biopsy?

Hello, recently I am having some difficulties with maintaining the cell culture of primary cells isolated from a skin biopsy (normal and malignant). The coating of culture plates with Matrigel (a...

26 February 2021 6,151 2 View