I want to confirm the Azotobacter chroococcum by sequencing it with normal 16s primers, so is there any general sequence available to be used as a reference sequence?
I run this gel(1%) and expecting a band of 730bp, but I can't figure out the size of band in the well marked by yellow arrow... so is this the band that I am expecting?
07 August 2018 7,870 2 View
I want to grow azotobacter chroococum, but I found 2 types of colonies...1.crusty and 2.watery (after 10 days incubation). So i separated them and plated on 2 different plates, so only crusty...
04 May 2018 2,072 0 View
I am getting smear in the gel of my pcr of CDNA samples, and to surprice me, I am getting band in GAPDH +ve control but not in my CDNA samples... Also I incorporated 2 plasmids as +ve controls,...
11 December 2017 710 2 View
I did Western blot of samples from cotton root tissues from same event, but of different time spans (took samples after 1 month interval for 4 months), and I got difference in banding...
06 July 2014 9,384 7 View
I have a dataset with about 80 different species. As usual, some species are very easy to identify with certainty whereas others are more difficult, which means that I am less certain of my...
03 March 2021 8,066 4 View
So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...
02 March 2021 1,146 2 View
I have to amplify a gene and my primers just reached. The Tm for Forward primer is 64.2, and that of reverse primer is 65.5. Can some one suggest how to get the best annealing temperature? Thanks...
01 March 2021 360 7 View
I am trying to identify these 3 genes among some tomato cultivar collections and after aligning some sequences from NCBI, I couldn't find unique sequences to target for specific primers. There...
28 February 2021 606 3 View
hello everyone, I need to do standard curves for my qPCR, what is the ideal efficiency range? I tried a primer (Mglu2 receptor) that gave an efficiency of 90.2%. Is it accepted?
28 February 2021 1,254 3 View
Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.
28 February 2021 5,008 4 View
Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...
26 February 2021 5,029 2 View
Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...
25 February 2021 6,969 3 View
Hi all, I am doing a genescan analysis for a deleted exon in the patient sample. The amplified region is 215 bp. In the patient sample because of homozygous deletion, no peak is observed while in...
23 February 2021 5,645 1 View
I am working on a CFX 96 machine to determine reaction efficiency of my gene of interest. After performing a serial dilution of my cDNA template, I was able to find great reaction efficiencies for...
23 February 2021 809 3 View